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Isolation of novel variants of infectious bursal disease virus from different outbreaks in northeast India. Classification of infectious bursal disease virus intogenogroups. Molecular genotyping of the infectious bursal disease virus (IBDV) isolated from broiler flocks in Egypt. In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE, editors. Phylogenetic analysis of infectious bursal disease viruses according to newly proposed model of classification into geno-groups. Sequence analysis of the VP2 gene hypervariable region of infectious bursal disease viruses from India. Restriction fragment length polymorphism in the VP2 gene of infectious bursal disease viruses from outside the United States. A proposed nomenclature for infectious bursal disease virus isolates. Molecular characterization of two Bangladeshi infectious bursal disease virus isolates using the hypervariable sequence of VP2 as a genetic marker. The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan. Novel variant infectious bursal disease virus suppresses Newcastle disease vaccination in broiler and layer chickens. Identification and characterization of a novel infectious bursal disease virus from outbreaks in Maharashtra province of India. In conclusion, both point mutation and genetic reassortment with intermediate type of vaccines were found to be responsible for generation of novel vvIBDV strains in this area which belonged to G3a and G3b genogroups.Īwandkar SP, et al. Additional changes at 270 (3 sequences) and 272 positions (4 sequences) could be attributed to reverse mutation or recombination with vaccine strains.
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In sequences from Maharashtra (group 2 of G3a lineage), occurrence of V instead of P/T/A at 222 position was recorded as a novel and conspicuous substitution in the immunodominant peak A of VP2 hypervariable region. Further divergence from prototypic vvIBDV strains was revealed as, D-N at 212 position (N = 9) and 279 position (N = 1). These sequences revealed important differences at key amino acid positions with respect to classical (G1 genogroup), variant (G2 genogroup) type of IBDV and classical vaccines. Considering the new genogrouping pattern, nine and four sequences of this study fell within G3a and G3b lineage, respectively. Upon nucleotide sequencing of amplicon and its deduction into amino acids, it was found that all the sequences of present study were related to vvIBDV according to old classification pattern. Among these 21 positive flocks, 11 (52.38%) were already vaccinated.
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Out of 54 bursa samples, 21 (38.89%) yielded the expected amplicon of 743 bp (701–1444 bp), and were found positive for IBDV. In the present study, suspected samples of IBD were collected from poultry flocks of districts of Gujarat and Nagpur (Maharashtra), identified using PCR and grouped as per traditional and new genogrouping pattern. Infectious bursal disease (IBD), caused by infectious bursal disease virus ( IBDV), has recently been reported in chickens vaccinated with classical or intermediate types of vaccines from various regions of India due to the emergence of novel very virulent strains of infectious bursal disease virus (vvIBDV).